Using Superfolder Green Fluorescent Protein for Periplasmic Protein Localization Studies
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| Authors: | Thuy Dinh Thomas G. Bernhardt |
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| Affiliation: | Department of Microbiology and Immunobiology, Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115 |
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| Abstract: | Studies investigating the subcellular localization of periplasmic proteins have been hampered by problems with the export of green fluorescent protein (GFP). Here we show that a superfolding variant of GFP (sfGFP) is fluorescent following Sec-mediated transport and works best when the cotranslational branch of the pathway is employed. |
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