Strategy for Modular Tagged High-Throughput Amplicon Sequencing |
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Authors: | Daniel Aguirre de C��rcer Stuart E. Denman Chris McSweeney Mark Morrison |
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Affiliation: | CSIRO Preventative Health National Research Flagship and Livestock Industries, Queensland Bioscience Precinct, St. Lucia, QLD 4067, Australia |
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Abstract: | The use and validation of a strategy that allows a universal set of bar-coded sequencing primers to be appended to an amplified PCR product is described. The strategy allows a modular approach, in that the same bar code can be used with two or more target-specific primer sets, even simultaneously. |
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