Genome-wide identification,characterization and expression analysis of the LIM transcription factor family in quinoa |
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| Authors: | Xiaolin Zhu Baoqiang Wang Xian Wang Chaoyang Zhang Xiaohong Wei |
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| Affiliation: | 1.College of Agronomy, Gansu Agricultural University, Lanzhou, 730070 China ;2.Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, 730070 China ;3.College of Life Science and Technology, Gansu Agricultural University, Lanzhou, 730070 China |
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| Abstract: | Lim family members play an important role in the regulation of plant cell development and stress response. However, there are few studies on LIM family in quinoa. In this study, we identified nine LIMS (named cqlim01-cqlim09) from quinoa, which were divided into three subfamilies (α Lim1, γ lim2 and δ lim2) according to phylogeny. The differences in gene structure and motif composition among different subfamilies have been observed. In addition, we studied the repetitive events of the members of the family. The Ka/Ks (non synchronous substitution rate / synchronous substitution rate) ratio analysis showed that the repetitive CqLIMs probably experienced purifying selection pressure. Promoter analysis showed that the family genes contained a variety of hormones, stress and tissue-specific expression elements, and protein interactions showed that these genes had actin stabilizing effect. In addition, QRT PCR results showed that all CqLIM genes were positively regulated under three stresses (low temperature, salt and ABA). These results provide a theoretical basis of further study of LIM gene in quinoa. Supplementary informationThe online version contains supplementary material available at 10.1007/s12298-021-00988-2. |
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| Keywords: | Quinoa LIM transcription factor family Bioinformatics analysis Gene expression |
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