Strategies for the production of lipase by Candida rugosa; neural estimation of biomass and lipase activity |
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| Authors: | Y Wang J Luopa T Rajalahti S Linko |
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| Institution: | (1) Laboratory of Biotechnology and Food Engineering, Department of Chemical Engineering, Helsinki University of Technology, FIN-02150 Espoo, Finland;(2) Present address: Michigan Biotechnology Institute, 3900 Collins Road, 48910 Lansing, Michigan, USA |
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| Abstract: | Summary Various strategies for the production of lipase by Candida rugosa Diddens and Lodder (ATCC 14830) in stirred tank reactors were investigated. The strain was first screened for lipase production with agar plate assay based on the fluorescent dye Rhodamine B, followed by adaptation to the production medium used. The highest lipase activity of 7.6 U/ml was obtained on a medium containing 40 g olive oil/l and 1 g glucose/l, with supplying pure oxygen. Neural networks were used in the estimation of biomass and lipase activity. |
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