Miscibility of acyl-chain defined phosphatidylcholines with N-palmitoyl sphingomyelin in bilayer membranes |
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Authors: | Bohdana Té rová |
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Affiliation: | Department of Biochemistry and Pharmacy, ÅBO AKADEMI UNIVERSITY, P.O. Box 66, FIN 20521 Turku, Finland |
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Abstract: | In this study we have used differential scanning calorimetry (DSC) to study the miscibility of different saturated phosphatidylcholines (PCs) with d-erythro-N-palmitoyl-sphingomyelin (16:0-SM). Information about the miscibility was obtained by observing the thermotropic phase behavior of binary mixtures of saturated PCs and 16:0-SM. The results obtained showed that PC miscibility in 16:0-SM was markedly affected by the PC acyl-chain composition. According to phase diagrams prepared from DSC data and the mid-transition temperatures of the main phase transition, the PC which formed the most ideal mixture with 16:0-SM was di-14:0-PC. However, the cooperativity of the main transition in PC/16:0-SM bilayers increased until the average acyl-chain length in the PC reached 15 carbons. Based on the criteria of the most ideal miscibility or the highest cooperativity of the main transition, we conclude that di-14:0-PC, 15:0/15:0-PC, and 14:0/16:0-PC interacted most favorably with 16:0-SM in bilayer membranes. Di-16:0-PC, to which 16:0-SM is often compared in biophysical studies, showed much less ideal miscibility. |
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Keywords: | 16:0-SM, smallcaps" >d-erythro-N-palmitoyl-sphingomyelin SM, sphingomyelin PC, phosphatidylcholine DSC, differential scanning calorimetry Tm, mid temperature of the gel to liquid-crystalline phase transition T1/2, half-width of the gel to liquid-crystalline phase transition XSM, the molar fraction of sphingomyelin |
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