Estrogen receptor purification by affinity chromatography using an orange triazine dye |
| |
Authors: | J P Bond A C Notides |
| |
Affiliation: | Department of Biophysics, University of Rochester School of Medicine and Dentistry, New York 14642. |
| |
Abstract: | A rapid two-step procedure was devised for the purification of the estrogen receptor from the calf uterus. A 900- to 1700-fold purification of the estrogen receptor was obtained using ammonium sulfate precipitation followed by dye affinity chromatography with Reactive Orange 14 immobilized to Sepharose. The Reactive Orange 14-Sepharose was used to purify the estrogen receptor in the presence or absence of estradiol as well as to purify the progesterone receptor. The purified estrogen receptor retained its estradiol- and DNA-binding properties and sedimented into sucrose gradients as the 5 S receptor dimer. The Reactive Orange 14-Sepharose is easily prepared and offers a higher yield and purity of the estrogen receptor than that afforded by estrogen- or heparin-Sepharose chromatography. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |