Silencing of filamin A gene expression inhibits Ca2+ -sensing receptor signaling |
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| Authors: | Huang Chunfa Wu Zhenzhen Hujer Kristine M Miller R Tyler |
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| Institution: | Division of Nephrology, Department of Medicine, Case Western Reserve University, Louis Stokes Veteran Affairs Medical Center, 10701 East Blvd., 151W, Cleveland, OH 44106, USA. chunfa.huang@case.edu |
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| Abstract: | Filamin plays an important role in actin cytoskeleton organization, membrane stabilization, and anchoring of transmembrane proteins. Using short interfering RNA (siRNA) to selectively target the filamin A gene and silence its expression, we studied the role of filamin A in G protein coupled receptor (GPCR) signaling. Silencing of filamin A protein expression was determined by immunoblotting and immunofluorescence. Functional consequences of filamin A gene silencing were measured by studying its role in MAPK signaling pathways activated by the Ca2+ -sensing receptor. This work defines filamin A involvement in GPCR signaling pathways and describes an additional method for studying its function. |
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| Keywords: | GPCR G protein coupled receptor HEK human embryo kidney CaR Ca2+-sensing receptor SAPK/JNK stress-activated protein kinases/c-Jun N-terminal kinase ERK extracellular signal-regulated kinase SEK1 SAPK/ERK kinase 1 MAPK mitogen-activated protein kinase |
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