Expression of a Streptomyces plasmid promoter in Escherichia coli |
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| Authors: | Z Deng T Kieser D A Hopwood |
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| Institution: | 1. School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, Zhejiang, China;2. Kentville Research and Development Centre, Agriculture and Agri-Food Canada, 32 Main Street, Kentville, NS B4N 1J5, Canada;3. Laboratory of aquatic product processing and quality safety, Marine Fisheries Research Institute of Zhejiang Province, Zhejiang, China |
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| Abstract: | A 166-bp DNA fragment from the Streptomyces multicopy plasmid pIJ101 with in vivo promoter activity both in Streptomyces lividans and in Escherichia coli was isolated. The start point of the RNA transcribed from this fragment, determined by high resolution S1 nuclease mapping, was the same in S. lividans and in E. coli. This suggests that the E. coli RNA polymerase recognizes the same sequence determinants and chooses the point of initiation of RNA synthesis in the same way as the corresponding S. lividans enzyme. The putative promoter sequence shows good homology to the E. coli promoter consensus sequence in the '-35' region but poor homology in the '-10' region. |
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