Synthesis,Characterization, Molecular Modeling,and DNA Interaction Studies of Copper Complex Containing Food Additive Carmoisine Dye |
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| Authors: | Nahid Shahabadi Alireza Akbari Mina Jamshidbeigi Reza Khodarahmi |
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| Affiliation: | 1. Inorganic Chemistry Department, Faculty of Chemistry, Razi University, Kermanshah, Iran;2. Medical Biology Research Center (MBRC), Kermanshah University of Medical Sciences, Kermanshah, Iran;3. Chemistry Department, Payame Noor University, Tehran, Iran;4. Medical Biology Research Center (MBRC), Kermanshah University of Medical Sciences, Kermanshah, Iran |
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| Abstract: | A copper complex of carmoisine dye; [Cu(carmoisine)2(H2O)2]; was synthesized and characterized by using physico-chemical and spectroscopic methods. The binding of this complex with calf thymus (ct) DNA was investigated by circular dichroism, absorption studies, emission spectroscopy, and viscosity measurements. UV-vis results confirmed that the Cu complex interacted with DNA to form a ground-state complex and the observed binding constant (2× 104 M?1) is more in keeping with the groove bindings with DNA. Furthermore, the viscosity measurement result showed that the addition of complex causes no significant change on DNA viscosity and it indicated that the intercalation mode is ruled out. The thermodynamic parameters are calculated by van't Hoff equation, which demonstrated that hydrogen bonds and van der Waals interactions played major roles in the reaction. The results of circular dichroism (CD) suggested that the complex can change the conformation of DNA from B-like form toward A-like conformation. The cytotoxicity studies of the carmoisine dye and its copper complex indicated that both of them had anticancer effects on HT-29 (colon cancer) cell line and they may be new candidates for treatment of the colon cancer. |
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| Keywords: | Carmoisine food additive Azo dye copper complex cytotoxicity DNA-groove binding hydrogen bond multispectroscopic methods van der waals interactions |
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