Correlation between pathogenicity and molecular characteristics in the willow leaf rusts <Emphasis Type="Italic">Melampsora epitea</Emphasis> and <Emphasis Type="Italic">M. humilis</Emphasis> in Japan |
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Authors: | Hitoshi Nakamura Shigeru Kaneko Yuichi Yamaoka Makoto Kakishima |
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Institution: | (1) Institute of Agriculture and Forestry, University of Tsukuba, Ibaraki, Japan, JP;(2) Forestry and Forest Products Research Institute, Ibaraki, Japan, JP |
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Abstract: | We studied the correlation between pathogenicity and restriction fragment length polymorphism (RFLP) type, which was determined
by polymerase chain reaction-based RFLP analysis of the internal transcribed spacer regions of ribosomal DNA, in the willow
leaf rust fungi Melampsora epitea and M. humilis. Eighteen clones of eight Salix species were inoculated with urediniospores from seven collections of the two rust species. M. epitea and M. humilis (RFLP type-5 collections) were pathogenic to six to eight Salix species. RFLP type-7 collections of M. epitea were pathogenic to only two Salix species. The taxonomic relationships of the two rust species are discussed.
Received: December 11, 2002 / Accepted: February 17, 2003
RID="*"
ID="*" Contribution no. 179, Laboratory of Plant Parasitic Mycology, Institute of Agriculture and Forestry, University of
Tsukuba, Japan
Acknowledgments We thank K. Katsuya, former professor at the University of Tsukuba, for his encouragement in this study. We are also grateful
to M. Yashima, Botanical Garden, University of Tohoku, for his assistance in collecting materials and to R. Suzuki, University
of Tsukuba, for providing a rust isolate. |
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Keywords: | Inoculation Melampsora epitea Melampsora humilis Pathogenicity Willow leaf rust |
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