4种染色方法对甜瓜白粉病菌染色效果的观察比较

考马斯亮蓝组织透明染色方法可以清楚观察到白粉病菌的5个发育阶段,即萌发的分生孢子、初生芽管、胞间菌丝、分生孢子梗和后期菌落。考马斯亮蓝几乎使寄主组织不着色,不产生背景色干扰,而菌体变深蓝色。苯胺蓝组织透明染色方法也可观察到病菌的不同发育阶段,但苯胺蓝易使寄主组织产生浅蓝色背景,而菌体呈现深蓝色,观察效果不理想。荧光素钠和苯胺蓝两种荧光染色方法均能使菌体在紫外或者蓝光下产生黄绿色荧光,而寄主组织呈现黑色背景,强烈的反差利于观察。荧光素钠可以观察到菌体整个发育阶段。苯胺蓝只适合于前期菌体入侵过程的观察。

Comparison of staining effects of four staining techniques for observation of Sphaerotheca fuliginea

Commassie brilliant blue staining can clearly display the different structures of Sphaerotheca fuliginea, such as germinating conidia, initial germtubes, hyphae and conidiophores, and hardly stain the host tissue, presenting a striking contrast between fungus and host tissue. Aniline blue staining can stain the host tissue, forming a light blue background, which results in unsatisfactory observation of fungal structures. Fluorescence staining with uranine and aniline blue generated yellow-green fluorescence in the fungal structures under UV or blue light, and the black background of host tissues gave rise to strong contrast for observation. The uranine staining can be used to observe the whole growing phases of Sphaerotheca fuliginea, while aniline blue staining is suitable for conidial observation.