Comparison of miRNA expression patterns using total RNA extracted from matched samples of formalin-fixed paraffin-embedded (FFPE) cells and snap frozen cells |
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| Authors: | Jinghuan Li Paul Smyth Richard Flavin Susanne Cahill Karen Denning Sinead Aherne Simone M Guenther John J O'Leary Orla Sheils |
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| Affiliation: | (1) Deptment of Histopathology, University of Dublin, Trinity College, Dublin, Ireland;(2) Applied Biosystems, Foster City, CA, USA |
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| Abstract: | Background Archival formalin-fixed paraffin-embedded (FFPE) tissues have limited utility in applications involving analysis of gene expression due to mRNA degradation and modification during fixation and processing. This study analyzed 160 miRNAs in paired snap frozen and FFPE cells to investigate if miRNAs may be successfully detected in archival specimens. |
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