Discarding duplicate ditags in LongSAGE analysis may introduce significant error |
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| Authors: | Jeppe Emmersen Anna M Heidenblut Annabeth Laursen Høgh Stephan A Hahn Karen G Welinder Kåre L Nielsen |
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| Institution: | (1) Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Aalborg, Denmark;(2) Department of Internal Medicine, Knappschaftskrankenhaus, University of Bochum, Bochum, Germany;(3) Department of Molecular Oncology, Weatherall Institute of Molecular Medicine, Oxford University John Radcliffe Hospital, Oxford, UK |
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| Abstract: | Background During gene expression analysis by Serial Analysis of Gene Expression (SAGE), duplicate ditags are routinely removed from
the data analysis, because they are suspected to stem from artifacts during SAGE library construction. As a consequence, naturally
occurring duplicate ditags are also removed from the analysis leading to an error of measurement. |
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