粘虫颗粒体病毒的增效因子提高杆状病毒的感染

一株美洲粘虫GV(增效品系)在东方粘虫幼虫上进行增殖，所获粘虫GV(PuGv-Ps>对东方粘虫NPV(PsNPV)、棉铃虫NPV(HaNPV)和黄地老虎NPV(AsNPV)进行增效试验。 实验结果证明PuGV-Ps对三种NPV都有明显的增效作用， 其中以对PsNPV为最强，增效率达55%一85%；对HaNPV为30%-80%；而对AsNPV只有15%-35%。用凝胶过滤技术从PuGV-Ps中分离增效因子PusF-Ps，其对PsNPV和A，NPV的增效作用亦同样明显，250μg的PuSF-Ps可以提高PsNPV的感染能力达80%。经ScPhadcf-150柱(1.6X 90)和使用四种标准蛋白(细胞色素c、胃蛋白酶、牛血清清蛋白和乳酸脱氢酶)测得PuSF-Ps的分子量为160 000左右。

ENHANCEMENT OF BACULOVIRUS INFECTION BY THE SYNERGISTIC FACTOR OF A GRANULOSIS VIRUS OF THE ARMYWORM

We have propagated Pseudaletis unipuncta granulosis virus (synergistic strain) in larvae of Pseudaletis separata and have conducted quantitative evaluation of the enhancement of the P. unipuncta granulosis virus-Ps (PuGV-Ps) on the NPVs of P. separata (Ps), Heliothis armigera (Ha) and Agrotis segetum (As). The results show the PuGV-Ps enhanced PsNPV, HaNPV and AsNPV infections in their original host insects. The high proportions (55%-85%, 30%-80%) of enhancement were observed in the PsNPV and HaNPV assays, and the median one (15%-35%) was obtained in the AsNPV-PsGV system. A synergistic factor (PuSF-Ps) has been isolated from the PuGV-Ps system using gel filtration technique. When PsNPV polyhedra were mixed with 250 ng PuSF-Ps, the value of enhancement increased by about 80%. The molecular weight of the synergistic factor was determined with Sephadex 150 column (1.6cm× 90cm) to be about 160k.