TGF-β1对AGS细胞上皮-间充质转化及体外侵袭的影响

目的观察转化生长因子-β1(TGF-β1)对人胃癌细胞株AGS发生上皮-间充质转化(epithelial-mesenchymal transition,EMT)及体外侵袭的影响。方法将体外培养的AGS用TGF-β1干预后,倒置显微镜下观察细胞形态学的变化,MTT比色法检测TGF-β1对AGS增殖的影响,细胞划痕试验和Transwell侵袭试验检测细胞运动和侵袭力的改变;免疫荧光和Western blot检测snail、E-cadherin(上皮钙粘蛋白)、和N-cadherin(神经钙粘蛋白)表达的变化。结果TGF-β1诱导AGS向间充质细胞形态转化,低浓度促进细胞增殖,而高浓度时细胞增殖率逐步降低,且snail和间充质细胞表型N-cadherin表达上调,而上皮细胞表型E-cadherin表达下调,同时细胞运动和侵袭能力大大增强。结论TGF-β1可诱导AGS发生EMT,从而增加其侵袭、转移的能力。

EFFECT OF TGF-B1 ON EPITHELIAL-MESENCHYMAL TRANSITION AND INVASION ABILITY OF AGS CELLS

Objective To investigate the effect of tranforming growth factor beta-1（TGF-β1） on epithelial-mesenchymal transition（EMT） and invasion ability of human gastric adenocarcinoma cell line AGS in vitro.Methods AGS cells were cultured with different concentratins of TGF-β1,The morphological change was observed under phase-contrast microscopy;the effect on proliferation of AGS was detected by MTT;the motility and invasion ability were examined by cell scratch test and transwell chamber model.The expressions of Snail,E-cadherin and N-cadherin were analyzed by immunofluorescence and Western blotting.Results TGF-β1 induced morphological alteration from epithelial to mesenchymal cells.Low concentrations of TGF-β1 accelerated AGS cell proliferation,and high concentrations restrained AGS proliferation.The expressions of Snail and mesenchymal cell marker N-cadherin were increased,while the expression of epithelial cell marker E-cadherin was decreased.The motility and invasion ability of AGS cells were greatly enhanced.Conclusion TGF-β1 induces epithelial mesenchymal transition of AGS cells,and their motility and invasion ability are enhanced.