A selective differential medium for the isolation of Listeria monocytogenes |
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| Authors: | G.D.W. Curtis R.G. Mitchell Andrea F. King Emma J. Griffin |
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| Affiliation: | Bacteriology Department and Regional Public Health Laboratory, John Radcliffe Hospital, Oxford OX3 9DU, UK |
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| Abstract: | A new medium has been developed for the isolation of Listeria monocytogenes from clinical specimens with a mixed flora. Almost complete inhibition of unwanted organisms was achieved and recognition of colonies of Listeria spp. was usually possible after 24 h using the aesculin-ferric ammonium citrate indicator system. Compared to McBride agar the new medium was more inhibitory to representative contaminating species in pure culture and more successful in isolating small numbers of L. monocytogenes from artificially seeded clinical specimens. |
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