|
|||||
|
|
Evidence of alterations in the expression of orphan receptors GPR26 and GPR39 due to the etiology of the metabolic syndrome |
|
| Authors: | Rodrigo Romero-Nava De-Shan Zhou Noemí García Armando Ruiz-Hernández Yin-Chu Si Fausto Sánchez-Muñoz |
| Institution: | 1. Escuela Superior de Medicina del Instituto Politécnico Nacional, Laboratorio de Se?alización Intracelular, Sección de Posgrado, Mexico;2. Department of Histology and Embryology, Capital Medical University, Beijing, China;3. Escuela Nacional de Medicina, Tecnológico de Monterrey, Monterrey, NL, Mexico;4. Centro de Investigación Básica y de Transferencia, Hospital Zambrano Hellio, Garza García, NL, Mexico;5. Department of Anatomy, Beijing University of Chinese Medicine, Beijing, China;6. Departamento de Inmunología, Instituto Nacional de Cardiología Ignacio Chávez, Ciudad de México, Mexico |
| Abstract: | Aims: Metabolic syndrome (MS) is composed of several metabolic abnormalities that increase the risk of cardiovascular diseases and diabetes. Although there are treatments for the components of MS, this pathology maintains a high mortality, suggesting that there are other mechanisms in which orphan receptors such as GPR26 and GPR39 may be involved. For this reason, the aim of this work was to evaluate the expression of GPR26 and GPR39 orphan receptors in two models of MS (diet and genetics). Materials and methods: We used male Wistar rats, which received 70% fructose in drinking water for 9 weeks, and obese Zucker rats. We measured weight, blood pressure, glucose, triglycerides, total cholesterol, HDL cholesterol, LDL cholesterol to determine the MS and the expression of the orphan receptors GPR26 and GPR39 in brain, heart, aorta, liver, and kidney by RT-PCR. Results: The analysis of the expression of the orphan receptors GPR26 and GPR39 showed that the receptors are expressed in some tissues, but the expression of the GPR26 tends to decrease in the heart and aorta, whereas in the brain, no changes were observed, this receptor is not expressed in the liver and kidney of both strains. The expression of GPR39 isoforms depends on the tissue and MS model. Conclusions: We conclude that the orphan receptors GPR26, GPR39v1, and GPR39v2 are expressed in different tissues and their profile expression is dependent on the etiology of the MS. |
| Keywords: | Metabolic syndrome orphan receptors GPR26 GPR39V1 GPR39V2 |