Preparation of tri(ethylene glycol) grafted core‐shell type polymer support for solid‐phase peptide synthesis |
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Authors: | Jaehi Kim Seojung Kim Dong‐Sik Shin Yoon‐Sik Lee |
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Institution: | 1. School of Chemical and Biological Engineering, Seoul National University, Seoul, Republic of Korea;2. Department of Chemical and Biological Engineering, Sookmyung Women's University, Seoul, Republic of Korea |
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Abstract: | A core‐shell type polymer support for solid‐phase peptide synthesis has been developed for high coupling efficiency of peptides and versatile applications such as on‐bead bioassays. Although various kinds of polymer supports have been developed, they have their own drawbacks including poor accessibility of reagents and incompatibility in aqueous solution. In this paper, we prepared hydrophilic tri(ethylene glycol) (TEG) grafted core‐shell type polymer supports (TEG SURE) for efficient solid‐phase peptide synthesis and on‐bead bioassays. TEG SURE was prepared by grafting TEG derivative on the surface of AM PS resin via biphasic diffusion control method and subsequent acetylation of amine groups which are located at the core region of AM PS resin. The performance of TEG SURE was evaluated by synthesizing several peptides. Three points can be highlighted: (1) easy control of loading level of TEG, (2) improved efficiency of peptide synthesis compared with the conventional resins, and (3) applicability of on‐bead bioassays. |
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Keywords: | core‐shell type resin on‐bead bioassay polymer supports solid‐phase peptide synthesis tri(ethylene glycol) |
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