Impact of formulation procedures on the effect of the biocontrol agent Serratia plymuthica HRO-C48 on Verticillium wilt in oilseed rape |
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Authors: | Henry Müller Gabriele Berg |
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Institution: | (1) Institute of Environmental Biotechnology, Graz University of Technology, Petersgasse 12, 8010 Graz, Austria |
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Abstract: | Verticillium wilt is an important disease in oilseed rape with an increasing importance worldwide. Currently, there are no
methods available to suppress the pathogen. A biological protection strategy on the basis of the plant-beneficial bacterium
Serratia plymuthica HRO-C48 to control Verticillium
dahliae in oilseed rape was developed. Three different techniques to apply the biocontrol agent to seeds, namely pelleting, film
coating and bio-priming, were evaluated considering the influence on the control activity, cell stability during storage and
practical feasibility. Neither the treatment nor the inoculum density was found to influence the abundances of HRO-C48 in
the rhizosphere after 30 days. Serratia treatment using bio-priming and pelleting resulted in a statistically significant biocontrol in comparison to the non-bacterized
controls. Additionally, survival of HRO-C48 differed between treatments, and was the highest using bio-priming at 20°C, and
pelleting at 4°C. In conclusion, the procedure of bio-priming, which was developed in line with this study, resulted in a
stable and efficient formulation of S. plymuthica on rape seed. This technology opens a possibility to develop a commercial Serratia formulation to protect oilseed against V. dahliae. |
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Keywords: | Verticillium Oilseed rape Serratia plymuthica Biological control Bacterial formulation |
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