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Fertilization of rabbit ova and the role of ovum investments in the block to polyspermy
Authors:David H McCulloh  Robert J Wall  Herbert Levitan
Institution:1. Department of Zoology, University of Maryland, College Park, Maryland 20742 USA;2. Reproduction Laboratory, U.S. Department of Agriculture, ARS, Agricultural Research Center, Animal Science Institute, Beltsville, Maryland 20705 USA;1. Instituto de Ciencia y Tecnología Animal, Universitat Politècnica de València, Camino de Vera s/n, 46022 Valencia, Spain;2. GenPhySE, Université de Toulouse, INRA, ENVT, 31326 Castanet Tolosan, France;1. Clinic of Small Animal Surgery and Reproduction, Center for Clinical Veterinary Medicine, Faculty of Veterinary Medicine, Ludwig Maximilian University, Munich, Germany;2. Institute of Veterinary Anatomy, University of Munich, Munich, Germany;1. Animal Technology and Research Center (CITA-IVIA), Polígono La Esperanza nº 100, 12400 Segorbe, Castellón, Spain;2. Institute of Science and Animal Technology (ICTA), Universitat Politècnica de València, 46022 Valencia, Spain;1. Department of Natural Resource Management, South Dakota State University, Brookings, SD, 57007, USA;2. Department of Geography and Environmental Sustainability, University of Oklahoma, Norman, OK, 73019, USA
Abstract:The rabbit ovum seldom becomes polyspermic despite the presence of supernumerary sperm with easy access to the ovum plasma membrane. The purpose of this study was to characterize the role of ovum investments in blocking polyspermy. Ova were inseminated with capacitated sperm in vitro and were fertilized. Early stages of development were normal. The incidence of polyspermy was determined by cytological examination of fixed ova. The incidence of polyspermy increased following removal of both the zona pellucida and corona radiata but not following removal of only the corona radiata. These results suggest that the failure of supernumerary sperm to penetrate the ovum plasma membrane is at least in part due to the presence of the zona pellucida.
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