qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data |
| |
Authors: | Jan Hellemans Geert Mortier Anne De Paepe Frank Speleman Jo Vandesompele |
| |
Affiliation: | (1) Center for Medical Genetics, Ghent University Hospital, B-9000 De Pintelaan, Ghent, Belgium |
| |
Abstract: | Although quantitative PCR (qPCR) is becoming the method of choice for expression profiling of selected genes, accurate and straightforward processing of the raw measurements remains a major hurdle. Here we outline advanced and universally applicable models for relative quantification and inter-run calibration with proper error propagation along the entire calculation track. These models and algorithms are implemented in qBase, a free program for the management and automated analysis of qPCR data. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |