Assay and Relationship of HT-2 Toxin and T-2 Toxin Formation in Liquid Culture |
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Authors: | Frank N. Kotsonis and Robert A. Ellison |
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Abstract: | Both T-2 toxin and HT-2 toxin can be conveniently quantitated in crude extracts by using a combination of thin-layer chromatography and fluorodensitometry. This technique was used to follow the production of these toxins by liquid cultures of Fusarium poae (NRRL 3287). T-2 toxin was produced prior to HT-2 toxin and hexadeuterio-T-2 toxin was converted by the culture to trideuterio-HT-2 toxin. |
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