Methodology for HLA typing of amniotic fluid fetal cells (author's transl)

Determination of HLA antigens can be used for prenatal diagnosis of some congenital anomalies such as adrenal hyperplasia (21-hydroxylase deficiency). This necessitates rigourous HLA typing of fetal cells cultivated in vitro. The method we have developed utilizes microcytotoxicity and quantitative microabsorption tests which have been adapted to the types of cells found in these cultures.