The peroxidase-glucose oxidase system: A new method to determine glucose liberated by carbohydrate degrading soil enzymes

This paper describes a new, accurate method for determination of glucose in soil enzyme assays. The low limit of detection of glucose by the peroxidase-glucose oxidase (PGO) system is 0.004 mg glucose · g soil⁻¹. Comparisons of PGO with the anthrone and phenol-sulfuric acid methods revealed no differences in the detection of glucose standard solutions. By eliminating the use of large volumes of H₂SO₄, the PGO system reduces laboratory hazards. A portion of this work was supported by National Science Foundation Grant DEB-8004172 and a grant from the Miami University Faculty Research Committee.