Analysis of n-hexane, 2-hexanone, 2,5-hexanedione, and related chemicals by capillary gas chromatography and high-performance liquid chromatography |
| |
| Authors: | A A Nomeir M B Abou-Donia |
| |
| Affiliation: | 1. State Key Laboratory of Bioreactor Engineering and Shanghai Collaborative Innovation Centre for Biomanufacturing, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China;2. School of Chemical and Environmental Engineering, Shanghai Institute of Technology, 100 Haiquan Road, Shanghai 201418, China;1. Princeton Plasma Physics Laboratory, 100 Stellarator Road, Princeton, NJ 08543, USA;2. Oak Ridge National Laboratory, Oak Ridge, TN, USA;3. Princeton University, Princeton, NJ, USA;4. Columbia University, New York, NY, USA;5. Lawrence Livermore National Laboratory, Livermore, CA, USA;2. Department of Joint Research Center, Koshigaya Hospital, Dokkyo Medical University, Koshigaya, Japan;1. Guangdong Provincial Key Laboratory of Distributed Energy Systems, Dongguan University of Technology, Dongguan, Guangdong, China;2. Hawaii Natural Energy Institute, University of Hawaii, Honolulu, HI, USA;3. College of Energy, Xiamen University, Xiamen, China;1. Centre for Crystal Growth, Department of Physics, SSN College of Engineering, Kalavakkam 603110, India;2. Department of Physics, B.S. Abdur Rahman University, Chennai 600048, India;1. School of Functional Food and Wine, Shenyang Pharmaceutical University, Shenyang, 110016, People’s Republic of China;2. Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang, 110016, People’s Republic of China;3. Shenyang Pharmaceutical University, Shenyang, 110016, People’s Republic of China |
| |
| Abstract: | Analytical methods, using capillary gas chromatography and normal-phase high-performance liquid chromatography, were developed for the analysis of the neurotoxic chemicals n-hexane, 2-hexanone, and 2,5-hexanedione and their suspected metabolites. Two gas chromatographic methods, using a 50-m glass capillary OV 101 column and cyclohexane as an internal standard, were employed. In both methods, the injector and detector temperatures were 220 and 280 degrees C, respectively. In method I the following temperature program was used: isothermic at 50 degrees C for 30 min, followed by a temperature increase of 10 degrees C/min to a final temperature of 180 degrees C, which was then maintained for 7 min. This method was used to analyze the following compounds: n-hexane, 2,5-dimethylfuran, 2-hexanone, 3-hexanone, hexanal, 1-hexanol, 2-hexanol, 3-hexanol, 5-hydroxy-2-hexanone, gamma-valerolactone, 2,5-hexanedione, and 2,5-hexanediol. Method II, which was developed for n-hexane and eight of its more common metabolites, used the following temperature program: isothermic at 70 degrees C for 15 min, followed by a temperature increase of 40 degrees C/min to a final temperature of 220 degrees C, which was maintained for 5 min. A linear relationship between peak area and amount injected was observed over a 100-fold range. The minimum detectable amounts ranged from 0.05 to 1 microgram, depending on the compound. Normal-phase HPLC, using a 5-micron silica cartridge fitted into an RCM-100 radial-compression separation system, was utilized to analyze 2-hexanone and its metabolites 2,5-dimethylfuran, gamma-valerolactone, 5-hydroxy-2-hexanone, and 2,5-hexanedione.(ABSTRACT TRUNCATED AT 250 WORDS) |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
