A rapid method for analyzing the ligation products of synthetic oligodeoxyribonucleotides |
| |
| Authors: | Larry U L Tan Bernard R Glick |
| |
| Institution: | (1) Connaught Laboratories, 1755 Steels Ave., W., M2R 3T4 Willowdale, Ontario, Canada;(2) Department of Biology, University of Waterloo, N2L 3G1 Waterloo, Ontario, Canada |
| |
| Abstract: | A method is described for the rapid analysis of DNA ligation products in the assembly of synthetic genes and gene fragments. The method is based on the simultaneous analysis of multiple ligation reactions where a single but different DNA oligomer is radiolabelled per ligation reaction. After each ligation the reaction mixture is electrophoresed on a denaturing, as well as a non-denaturing, polyacrylamide gel allowing one to monitor the ligation reaction products. In addition, a unique method for generating single stranded DNA sizing standards up to approximately 300 nucleotides in length is described. |
| |
| Keywords: | synthetic gene DNA analysis ligation polyacrylamide gel electrophoresis |
| 本文献已被 SpringerLink 等数据库收录！ |
|
