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淮河克氏原螯虾种群遗传结构
引用本文:李小花,刘刚,周立志,鲁的苗. 淮河克氏原螯虾种群遗传结构[J]. 动物学杂志, 2013, 48(6): 868-874
作者姓名:李小花  刘刚  周立志  鲁的苗
作者单位:安徽大学资源与环境工程学院 合肥 230601;安徽大学资源与环境工程学院 合肥 230601;安徽大学资源与环境工程学院 合肥 230601;安徽大学资源与环境工程学院 合肥 230601
基金项目:安徽省自然科学基金项目(No. 11040606M76),安徽大学大学生科研训练计划项目(No.KYXL20110041)
摘    要:克氏原螯虾(Procambarus clarkii)是广泛分布于我国淡水水域的外来入侵物种,对其种群遗传结构的研究有助于了解种群扩散过程。本文测定了淮河流域自然水体中9个地理种群共151个野生克氏原螯虾线粒体COⅠ序列,获得624 bp的有效基因片段,共定义了25个单倍型。AMOVA分析结果表明,淮河流域的克氏原螯虾种群内的变异占主导地位,单倍型多样性和核苷酸多样性均为中等水平,各地理种群间平均遗传差异较小(Fst=0.078),遗传差异不显著。系统发生树并没有按地理位置形成对应族群,表明克氏原螯虾各地理种群间存在明显的基因流,各地理种群间没有形成明显的种群遗传分化。

关 键 词:克氏原螯虾  线粒体细胞色素氧化酶Ⅰ  种群遗传结构  淮河
收稿时间:2013-01-27
修稿时间:2013-05-21

Population Genetic Structure of Red Swamp Crayfish in Haihe River Wetlands
LI Xiao-Hu,LIU Gang,ZHOU Li-Zhi and LU Di-Miao. Population Genetic Structure of Red Swamp Crayfish in Haihe River Wetlands[J]. Chinese Journal of Zoology, 2013, 48(6): 868-874
Authors:LI Xiao-Hu  LIU Gang  ZHOU Li-Zhi  LU Di-Miao
Affiliation:School of Resources and Environmental Engineering, Anhui University, Hefei 230061, China;School of Resources and Environmental Engineering, Anhui University, Hefei 230061, China;School of Resources and Environmental Engineering, Anhui University, Hefei 230061, China;School of Resources and Environmental Engineering, Anhui University, Hefei 230061, China
Abstract:Procambarus clarkia is an alien invasive crayfish species which widely distributed in the freshwater in China, to study the population genetic structure will help us to understand its spread process. Here, we analyzed the population genetic structure among 9 populations of 151 crayfish individuals in Huaihe river based on the COI region, the results showed the 624 bp long COI region aligned across 25 haplotypes; AMOVA shows that intra-population variation (92.20%) is much higher than inter-population variation (7.80%). Haplotype diversity and nucleotide diversity is moderate, and the differentiation among populations is non-significan (Fst=0.078). Phylogenetic tree shows that the genealogical clusters are not matched the sample locations well, suggesting that genetic differentiation among them is not remarkable, and the gene flow is remarkable.
Keywords:
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