Regulation of Sertoli cell cyclic adenosine 3':5' monophosphate phosphodiesterase activity by follicle stimulating hormone and dibutyrl cyclic AMP |
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| Authors: | M Conti R Geremia S Adamo M Stefanini |
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| Institution: | Istituto di Istologia ed Embriologia Generale, Università di Roma Via A. Scarpa 14, 00161 Roma, Italia |
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| Abstract: | Total phosphodiesterase activity was measured in Sertoli cell culture after exposure to isobutyl-methyl-xanthine, dibutyryl cyclic AMP and FSH. After 24 hr of incubation both FSH and dibutyryl cAMP caused a significant increase in total phosphodiesterase activity of Sertoli cell homogenates (control: 66 ± 16 pmoles/min/mg protein; FSH: 291 ± 25 pmoles/min/mg protein; dibutyryl cAMP: 630 ± 70 pmoles/min/mg protein). FSH stimulation was potentiated by isobutyl-methyl-xanthine. Both in the presence and absence of xanthine, the induction of phosphodiesterase was dependent on the FSH concentration, with maximal stimulation achieved with 0.5–1.0 μg FSH/ml. The induction of phosphodiesterase activity by hormone was abolished by cycloheximide treatment. The data suggest that FSH regulates phosphodiesterase activity via changes of cAMP levels in Sertoli cell in culture. |
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| Keywords: | MIX 3-isobutyl-1-methyl-xanthine db-cAMP 6-N 2-O-dibutyryl adenosine 3′:5′ monophosphate cAMP adenosine 3′:5′ cyclic monophosphate FSH Follicle Stimulating Hormone PMSF phenyl methyl sulfonyl fluoride BSA Bovine Serum Albumin ABP Androgen Binding Protein |
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