In vivo detection of nitric oxide distribution in mice |
| |
Authors: | Komarov Andrei M. |
| |
Affiliation: | (1) Department of Physiology and Experimental Medicine, The George Washington University Medical Center, Washington, DC, USA |
| |
Abstract: | This paper discusses in vivo detection of nitric oxide (NO) distribution in endotoxin-treated mice using L-band (1.1 GHz) electron paramagnetic resonance spectroscopy (EPR) in combination with the hydrophilic NO trapping complex: N-methyl-D-glucamine dithiocarbamate and iron (MGD-Fe). MGD-Fe-NO complex is found in the upper abdomen (liver region), lower abdomen (kidney and urinary bladder) and head region of ICR mice. Experiments with nitric oxide synthase (NOS) inhibition and 15N-labeled L-arginine as NOS substrate verify the origin of trapped NO from L-arginine. However, contribution from a 'nonenzymatic' NO generation pathway can not be ruled out. This paper further examines potential artifacts, which may arise in experiments using dithiocarbamate-iron complexes as NO trapping agents. |
| |
Keywords: | diethyldithiocarbamate endotoxic shock inducible nitric oxide synthase in vivo EPR nitric oxide spin trapping |
本文献已被 SpringerLink 等数据库收录! |
|