Ability of dialysates containing transfer factor to induce lymphocyte activating factor by human mononuclear cells. |
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| Authors: | A Togawa J J Oppenheim C H Kirkpatrick |
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| Institution: | 1. Laboratory of Microbiology and Immunology, National Institute of Dental Research, National Institute of Health, Bethesda, Maryland 20014 U.S.A.;2. Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institute of Health, Bethesda, Maryland 20014 U.S.A. |
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| Abstract: | Dialysates of human leukocyte lysates containing transfer factor (TFd) stimulated human mononuclear cells (MNL) to produce lymphocyte activating factor (LAF). Both unfractionated and adherent MNL cultures were stimulated by TFd to produce a factor which was mitogenic for murine thymocytes and had the biochemical characteristics of LAF as determined by Bio-Gel P-100, DEAE cellulose, and hydroxylapatite chromatography. Fractionation of TFd on Sephadex G-25 showed that the specific transfer factor activity of converting in vivo skin tests was present in the major uv-absorbing peak, whereas the substance(s) that induced LAF activity was present in a number of the other fractions. Therefore, the capacity of TFd to induce monocytes to produce LAF is not a measure of classical transfer factor activity. However, this effect of TFd may instead participate in the nonspecific immunoenhancing effects of TFd. |
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| Keywords: | Send reprint requests to Dr Joost J Oppenheim Bldg 30 Room 322 National Institute of Dental Research National Institutes of Health Bethesda Maryland 20014 |
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