Cell type-specific expressions of c-ras gene products in the normal rat |
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| Authors: | Takeo Tanaka Noriaki Ida Chiaki Waki Hiroko Shimoda Dennis J Slamon Martin J Cline |
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| Institution: | (1) Department of Pediatrics, Hiroshima University School of Medidcine, 734 Kasumi, Hiroshima, Japan;(2) Department of Pediatrics MAZDA Hospital, MAZDA Mortor Corporation, 2-15, Aosaki-minami, 735 Fuchu-cho, Aki-gun, Hiroshima, Japan;(3) Department of Pediatrics, Hiroshima University School of Medidcine, 734 Kasumi, Hiroshima, Japan;(4) Department of Pediatrics, Hiroshima University School of Medidcine, 734 Kasumi, Hiroshima, Japan;(5) Division of Hematology and Oncology, Department of Medicine, UCLA School of Medidcine, Los Angeles, CA 90024, USA;(6) Division of Hematology and Oncology, Department of Medicine, UCLA School of Medidcine, Los Angeles, CA 90024, USA |
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| Abstract: | Expression of proteins encoded by the ras proto-oncogenes was examined immunohistochemically in formalin-fixed, paraffin-embedded tissues of the normal rat using anti-ras p21 antibodies generated against synthetic peptides. Cell type specific expressions of ras gene products were detected in distal tubules of kidney, megakaryocytes in spleen, neural cells in cerebrum, Purkinje cells in cerebellum, cells lining the pulmonary alveoli and cells in the epithelium of intestinal villi. Region specific expressions of the ras proteins were observed in spleen and thymus, where the ras proteins were detected in splenic nodules including germinal centers and thymic medulla, respectively. These findings suggest that the c-ras gene products in normal rat organs are expressed in specific cell-types within a tissue and may be associated with degree of cellular differentiation. |
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| Keywords: | c-ras p21 normal rat organs immunohistochemistry Western blotting |
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