Mitochondria-derived and extra-mitochondrial human type-1 porin are identical as revealed by amino acid sequencing and electrophysiological characterisation |
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Authors: | Stadtmüller U Eben-Brunnen J Schmid A Hesse D Klebert S Kratzin H D Hesse J Zimmermann B Reymann S Thinnes F P Benz R Götz H Hilschmann N |
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Institution: | Max-Planck-Institut für experimentelle Medizin, Abteilung Immunchemie, G?ttingen, Germany. |
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Abstract: | In mammalian cells porin channels are localised in both mitochondrial outer membranes and extra-mitochondrial membranes. We isolated mitochondria-derived porin of a human lymphoblastoid B cell line, determined its amino acid sequence and characterised its channel properties. Interestingly, the amino acid sequence of this porin preparation and, correspondingly, its electrophysiological characteristics in a reconstituted system were identical to those of 'Porin 31HL', the human type-1 porin purified from a crude membrane preparation of the same cell line using a different purification protocol. The results raise questions about targeting, insertion and orientation of human type-1 porin in different membranes. |
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