| 乳糖代替IPTG诱导重组人胸腺肽α1在大肠杆菌中的表达 |
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| 引用本文： | 罗豪晖,蒋为民,李辰生.乳糖代替IPTG诱导重组人胸腺肽α1在大肠杆菌中的表达[J].微生物学免疫学进展,2010,38(2):32-35. |
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| 作者姓名： | 罗豪晖 蒋为民 李辰生 |
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| 作者单位： | 深圳市海王英特龙生物技术股份有限公司,深圳,518057 |
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| 摘 要： | 研究以乳糖代替IPTG作为诱导剂诱导重组人胸腺肽α1表达的可行性,对乳糖诱导的时机、乳糖浓度、诱导持续时间以及其它诱导条件进行研究,确定了乳糖诱导的最佳条件。结果表明,乳糖能有效地诱导重组人胸腺肽α1的表达,并且目的蛋白的表达量略高于IPTG的诱导量。
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| 关 键 词： | 胸腺肽α1 乳糖 诱导 IPTG |
Expression of recombinant human Thymosin alpha1 in Escherichia coli induced by lactose instead of IPTG |
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| LUO Hao-hui,JIANG Wei-min,LI Chen-sheng.Expression of recombinant human Thymosin alpha1 in Escherichia coli induced by lactose instead of IPTG[J].Progress In Microbiology and Immunology,2010,38(2):32-35. |
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| Authors: | LUO Hao-hui JIANG Wei-min LI Chen-sheng |
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| Institution: | ( Shenzhen Neptunus Interlong Bio-Tech Co. ,Ltd , Shenzhen S18057 , China) |
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| Abstract: | To study the feasibility of expression of Thymosin alphal induced by lactose instead of IPTG. The factors such as the optimal induction time, the optimal inducer concentration, the optimal induction duration and other induction conditions were studied, and the optimal induction conditions were determined. The results showed that Thymosin alphal is effectively induced by lactose, and the induction efficiency of lactose is better than that of IPTG. |
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| Keywords: | IPTG |
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