| 基于二次正交旋转回归优化大豆根内拮抗菌AF-67发酵工艺 |
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| 引用本文： | 区伟佳,王荣,潘剑文,李华兴.基于二次正交旋转回归优化大豆根内拮抗菌AF-67发酵工艺[J].生物数学学报,2010(4):707-712. |
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| 作者姓名： | 区伟佳 王荣 潘剑文 李华兴 |
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| 作者单位： | 华南农业大学资源环境学院,广东广州510642 |
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| 基金项目： | 国家自然科学基金项目（40971155）; 广东省科技攻关项目（2006B20301050）; 广东省教育部产学研结合项目（2009B090300330） |
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| 摘 要： | 应用二次正交旋转回归设计,研究培养基关键影响的四因子对大豆根腐生防菌AF-67发酵菌液浓度的影响.优化发酵培养基各成分的最佳配比为:牛肉浸膏1%,蛋白胨3.5%,可溶性淀粉1.8%,葡萄糖1%,发酵24h后稀释10倍工作液的实际光密度值A_(620)为0.743,较LB增殖培养液提高了42%.肯定了模型的可靠性和应用的有效性.
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| 关 键 词： | 正交旋转回归 发酵 大豆根腐病 |
Optimization of Fermentation for Antifungal Entophytic Bacteria AF-67 by Quadratic Orthogonal Regression Design |
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| OU Wei-jia,LI Hua-xing.Optimization of Fermentation for Antifungal Entophytic Bacteria AF-67 by Quadratic Orthogonal Regression Design[J].Journal of Biomathematics,2010(4):707-712. |
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| Authors: | OU Wei-jia LI Hua-xing |
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| Institution: | WANG Rong PAN Jian-wen (College of Natural Resources and Environment,South China Agricultural University,Guangzhou Guangdong 510642 China) |
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| Abstract: | The test was designed by using the Quadratic Orthogonal Rotation Combination Design,and the effect of various factors on response value for fermentation broth of AF-67 was analyzed.The results showed that ingredients of fermentation medium were optimized as follows： 1%beef extract,3.5%peptone,1.8%soluble starch and 1%glucose.Perform fermentation test by using the optimized medium,and the result showed that the mean A620 value of fermentation broth was 0.743,which was increased by 42%as compared with the proliferation of LB culture medium.The statistic test showed that the regression model is reliable and valid. |
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| Keywords: | Rotating orthogonal regression design Fermentation Soybean root rot |
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