| 微孔板与响应面相结合筛选壳聚糖酶高产菌株 |
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| 引用本文： | 赵华,任晶,王虹,陈磊.微孔板与响应面相结合筛选壳聚糖酶高产菌株[J].工业微生物,2011,41(3):12-16. |
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| 作者姓名： | 赵华 任晶 王虹 陈磊 |
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| 作者单位： | 工业微生物教育部重点实验室,天津科技大学生物工程学院,天津,300457 |
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| 摘 要： | 利用微孔板与响应面相结合筛选壳聚糖酶高产菌株.经平板透明圈法从土样中筛选出60株产壳聚糖酶菌株,再经96孔板复筛得到1株产壳聚糖酶活力较高的菌株.通过Plackett-Burman设计实验确定蛋白胨、胶体壳聚糖、CaCl2对产酶具有显著影响;最后应用中心组合设计和响应面分析得到以上3个因素的最佳浓度分别为(%,w/v)...
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| 关 键 词： | 壳聚糖酶高产菌 微孔板 响应面法 培养基 |
Optimization for screening high chitosanase-producing strain by microplate and Response Surface Methodology |
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| ZHAO Hua,REN Jing,WANG Hong,CHEN Lei.Optimization for screening high chitosanase-producing strain by microplate and Response Surface Methodology[J].Industrial Microbiology,2011,41(3):12-16. |
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| Authors: | ZHAO Hua REN Jing WANG Hong CHEN Lei |
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| Institution: | (Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science ＆ Technology, Tianjin 300457. China) |
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| Abstract: | Microplate combinded with Response Surface Methodology were used to screen high chitosanase-producing strain. 60 strains producing chitosanase were isolated from soil samples by plate screening, and then one of the higher chitosanase-producing strains was screened by 96 well plates-ELIASA Reader. Plaekett-Burman design was applied to evaluate three important factors affecting chitosanase production, which were colloid chitosan, pepeone and CaCl2. The optimal levels of the main factors were obtained by central composite design and response surface analysis, which were pepeone 1.12%, colloid chitosan 1.26%, and CaC12 0.91%. Under the optimal cultural conditions, the activity of chitosanase was 34.95 U/mL, which was 2.31 times higher than that of the initial strain. |
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| Keywords: | high chitosanase-producing strain microplate Response Surface Methodology medium |
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