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| 纳豆激酶发酵液的脱色工艺研究 | |
| 引用本文： | 董超,史延茂,杨明,王辉,马跃华.纳豆激酶发酵液的脱色工艺研究[J].工业微生物,2011,41(3):60-65. |
| 作者姓名： | 董超 史延茂 杨明 王辉 马跃华 |
| 作者单位： | 1. 河北省科学院生物研究所,石家庄,050081 2. 石药集团维生药业有限公司,石家庄,050035 3. 河北省科学院生物研究所,石家庄,050081;河北工业大学化工学院生物工程系,天津,300130 |
| 基金项目： | 河北省科技厅2008年科技支撑项目 |
| 摘 要： | 从11种待选脱色介质中筛选出330(OH)型树脂对纳豆激酶发酵液进行脱色研究.结果表明:(1)330(OH)型树脂对纳豆激酶发酵液中的色素为优吸型吸附,其动力学模型符合扩散方程,其动力学拟和方程为-ln(1-F)=0.0223t+0.0511,R2=0.9978,其中F=Qt/Qe,Qt为脱色时间为t时330(OH)型... |
| 关 键 词： | 纳豆激酶 色素 阴离子交换树脂 脱色 |
Studies on decolorization of nattokinase fermentation liquor |
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| DONG Chao,SHI Yan-mao,YANG Ming,WANG Hui,MA Yue-hua.Studies on decolorization of nattokinase fermentation liquor[J].Industrial Microbiology,2011,41(3):60-65. | |
| Authors: | DONG Chao SHI Yan-mao YANG Ming WANG Hui MA Yue-hua |
| Institution: | 1. Hebei Institute of Biology, Shijiazhuang 050081, China; 2. Hehei University of Technology, Tianjin 300130, China; 3. Weisheng Pharmaceutical (Shijiazhuang) Co. Ltd., Shijiazhuang 050035, China) |
| Abstract: | For decolorization of nattokinase fermentation liquor, resin 330（OH） was selected from 11 different decolorizing mediums. The results showed that （1）Resin 330 （OH）could effectively adsorb the coloring matters in nattokinase fermentation liquor. The kinetic model accorded to diffusing equation well. The kinetic Folynomial equation was -ln（ 1 - F） = 0. 0223 t ＋ 0.0511, R2= 0. 9978, in which t was decolonizing time. （2）Under static condition, the proportion of resin and fermentation liquor was 15 g： 40 mL at pH 7.0 for 200 min. The decolorization effidency was 90%, the activity of enzyme was 97.67 and the purification multiple was 3.09. （3）The dynamic condition consisted of pH 7.0, flow-velodty of 1.0 BV/h and operation time 5 h. The decolorizing liquor was mixed with eluant of phosphate buffer at pH 7.0 by flow-velocity 0.9 Bv. The total decolorization efficiency was 90 %, the activity of enzyme was 98 % and the purification multiple was 3.41. |
| Keywords: | nattokinase pigment negative ion exchange resin decoloration |
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