高产大黄素赭曲霉的离子注入诱变选育及发酵特性分析

通过N+离子注入获得大黄素高产菌株Aspergillus ochraceus LP-0301并优化了发酵条件,使其最终产率达1.453 mg/L,比出发菌株提高2.96倍.优化后的培养基为:镁离子1.5 g/L,硝酸铵10 g/L,蛋白胨10 g/L,玉米淀粉75 g/L;优化后的发酵条件为:温度30 ℃,初始pH为7...

Breeding of emodin-producing strains by N+ implantation and analysis of fermentative conditions and properties

The productivity of emodin was improved by N＋ low-energy ion beam irradiation and up to 1. 453 mg/L by optimization of medium and fermentation conditions. The optimized medium was composed of Mg2＋ （1.5 g/L）, ammonium nitrate （10 g/L）, peptone （10 g/L） and corn flour （75 g/L）. The optimized fermentation conditions were as follows： temperature 30℃, initial pH 7, media volume in flask 80 mL, inoculum rate 10 % and seed age 20 h. The results showed that the growth of LP-0301 and emodin production were separated obviously. The growth stage of LP-0301 was short and the myeelium was small. The stage of emodin production was long and the productivity of emodin was high. The content of emodin from LP-0301 was higher than those of from many traditional chinese medicines, so it was feasible to obtain emodin by LP-0301 fermentation.