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餐厨垃圾厌氧消化产沼气过程中酶学表征
引用本文:李永灿,严群,阮文权.餐厨垃圾厌氧消化产沼气过程中酶学表征[J].工业微生物,2011,41(3):76-80.
作者姓名:李永灿  严群  阮文权
作者单位:1. 江南大学生物工程学院,无锡,214122;江南大学环境与土木工程学院,无锡,214122
2. 军事医学科学院生物工程研究所,北京,100071
基金项目:国家科技支撑计划项目,国家自然科学基金项目,江苏省科技支撑项目,江南大学自主科研计划资助
摘    要:厌氧消化产沼气被认为是餐厨垃圾资源化利用的有效方式之一,其实质是在多种微生物综合作用下的生物化学过程.本文研究了在促进和抑制性因子作用下,餐厨垃圾厌氧发酵的酶学过程,对其中的脱氢酶和水解酶(β-葡萄糖苷酶,BAA-蛋白水解酶,碱性磷酸酶)活性变化进行了分析.研究表明,与空白对照组相比添加酵母粉后脱氢酶的最高活性提高了8...

关 键 词:厌氧发酵  餐厨垃圾  脱氢酶  水解酶

Enzymatic characterization of dehydrogenase and hydrolase during anaerobic digestion process from kitchen waste
LI Yong-can,YAN Qun,RUAN Wen-quan.Enzymatic characterization of dehydrogenase and hydrolase during anaerobic digestion process from kitchen waste[J].Industrial Microbiology,2011,41(3):76-80.
Authors:LI Yong-can  YAN Qun  RUAN Wen-quan
Institution:1. School of Biotechnology of Jiangnan University, Wuxi 214122; 2. School of Environment and Civil Engineering of Jiangnan University, Wuxi 214122; 3. Institute of Biotechnology, Academy of Military Medical,Beijing 100071)
Abstract:As one of the best utilization ways of kitchen wastes, methane production through anaerobic digestion was actually a synergctic biochemical process, which involved many micro-organisms. Enzymes activities, especially dehydrogenase and some hydrolases (β-glucosidase, EAA-hydrolysing production and phosphatase) during anaerobic digestion with yeast powder and chlorampherical were studied. Research results sh3wed that with the addition of yeast Fowder, compared with the control, the activity of dehydrogenase increased by 86%, and the production of methane increased by 100%, the activities of β-glucosidase and BAA-hydrolysing protease respectively increased by 46% and 62%. In contrast,compared with the control, with the addition of chloramphenicol, the activities of these three enzymes decreased by 31% ,22% and 35%, respectively.
Keywords:anaerobic digestion  kitchen waste  dehydrogenase  hydrolase
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