Characteristics of bacterium GB-2, a presumptiveCytophaga species with novel immunoregulatory properties |
| |
Authors: | Dr William R Usinger George C Clark Ellen Gottschalk Stanley Holt Robert I Mishell |
| |
Institution: | (1) Department of Microbiology and Immunology, University of California, 94720 Berkeley, California, USA;(2) Physiological-Chemical Institute, Göttingen, FRG;(3) Department of Periodontics, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA |
| |
Abstract: | A Gram-negative, psychrophilic bacterium, designated GB-2, was isolated from fetal calf serum and analyzed for its morphological, physiological, and biochemical characteristics. Gliding motility, sensitivity to actinomycin D, the presence of the pigment flexirubin and cytochrome c, growth on a variety of carbohydrates, the production of acid fermentation products, and a 34.9 mol% guanine+cytosine (G+C) content of bacterial DNA indicate GB-2 to be a member either of the generaCytophaga orFlexibacter. Growth of GB-2 was optimized in a simple defined medium to facilitate isolation and characterization of bacterial products. Liquid growth of GB-2 resulted in the release of significant quantities of a macromolecule free of both endotoxin and protein into the growth supernatant, which activated the proliferation of murine lymphocytes. The relationship between this bacterium and its end-products to other species of theCytophaga/Flexibacter group is discussed. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|