| 酵母三杂交系统的原理和应用 |
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| 引用本文： | 彭丹妮,黄静,吴自荣. 酵母三杂交系统的原理和应用[J]. 生命科学, 2007, 19(4): 461-464 |
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| 作者姓名： | 彭丹妮 黄静 吴自荣 |
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| 作者单位： | 华东师范大学生命科学学院,上海,200062 |
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| 基金项目： | 上海市科委重大计划项目 |
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| 摘 要： | 酵母双杂交系统自出现以来,广泛用于研究蛋白质之间的相互作用,它是一种具有高灵敏度的研究蛋白质之间关系的技术.在酵母双杂交系统基础上发展的酵母三杂交系统将应用范围扩展到蛋白质-蛋白质、蛋白质-RNA、蛋白质-小分子化合物等更广阔的研究领域.本文着重介绍酵母三杂交系统的原理、应用及局限性.
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| 关 键 词： | 酵母三杂交系统 蛋白质-蛋白质相互作用 蛋白质-RNA相互作用 蛋白质-小分子化合物相互作用 |
| 文章编号： | 1004-0374（2007）04-0461-04 |
| 收稿时间： | 2007-01-29 |
| 修稿时间： | 2007-04-18 |
Principle and application of the yeast three-hybrid system |
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| PENG Danni,HUANG Jing,WU Zirong. Principle and application of the yeast three-hybrid system[J]. Chinese Bulletin of Life Sciences, 2007, 19(4): 461-464 |
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| Authors: | PENG Danni HUANG Jing WU Zirong |
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| Affiliation: | School of Life Science, East China Normal University, Shanghai 200062, China |
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| Abstract: | The yeast two-hybrid system is widely employed in genetic screening and detecting protein-protein interactions. The yeast three-hybrid system, based on the yeast two-hybrid system, can be applied to much broader areas for detection of interactions between protein-protein, protein-RNA and protein-small ligand. This minireview deals with the principle, application, and shortcoming of the yeast three-hybrid system. |
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| Keywords: | yeast three-hybrid system protein-protein interactions protein-RNA interactions proteinsmall ligand interactions |
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