Mannose receptor dependent uptake of a ricin A chain--antibody conjugate by rat liver non-parenchymal cells |
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| Authors: | N R Worrell D N Skilleter A J Cumber R J Price |
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| Affiliation: | 1. Division of Biology, Institute of Cancer Research, Chester Beatty Laboratories, Fulham Road, London, S.W.3 UK;2. MRC Toxicology Unit, Medical Research Council Laboratories, Woodmansterne Road, Carshalton, Surrey, SM5 4EF UK;1. Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, People’s Republic of China;2. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, People’s Republic of China;1. Thoracic Oncology Department, Larrey Hospital, University Hospital of Toulouse, France;2. Inserm, Centre de Recherche en Cancérologie de Toulouse, CRCT UMR-1037, Toulouse, France;3. Laboratoire de Biologie Médicale Oncologique, Institut Universitaire du Cancer de Toulouse, France;4. University of Toulouse III (Paul Sabatier), Toulouse, France;5. Cellule Biostatistique, Bureau des Essais Cliniques, Institut Claudius Regaud, IUCT-Oncopole, Toulouse, France;6. Pathology Department, IUCT-Oncopole, Toulouse, France;1. Department of Biological Sciences, University of Ulsan, Ulsan, 680-749, South Korea;2. Department of Radiology, Kangbuk Samsung Hospital, Seoul, South Korea;3. Department of Surgery Ulsan University Hospital, University of Ulsan, Ulsan, South Korea;4. Meta-Inflammation Research Center, University of Ulsan, Ulsan, 680-749, South Korea |
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| Abstract: | Mannose receptor mediated uptake by the reticuloendothelial system has been suggested as an explanation for the rapid removal of ricin A chain antibody conjugates from the circulation after their administration. We have measured, in the rat, hepatic uptake of a ricin A chain antibody conjugate in vivo and its susceptibility to inhibition by a mannosylated protein and have measured uptake of the conjugate in vitro by rat parenchymal and non-parenchymal liver cells. The results indicate that rapid hepatic uptake of conjugate does occur in vivo; cultured non-parenchymal cells accumulate the conjugate to a much greater degree than cultured parenchymal cells and that mannose receptors appear to be involved in the process. |
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