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A rapid DNA extraction method for sugarcane and its relatives |
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| Authors: | Rhonda J. Honeycutt Bruno W. S. Sobral Paul Keim James E. Irvine |
| Affiliation: | (1) Texas A&M University Agricultural Research and Extension Center, 78596 Weslaco, TX, USA;(2) Present address: California Institute of Biological Research, 11099 North Torrey Pines Road, Suite 300, 92037 La Jolla, CA, USA;(3) Northern Arizona University, 201 Biology Building, South Beaver Street, 86011 Flagstaff, AZ, USA |
| Abstract: | A simple DNA extraction method based on CTAB precipitation was used to obtain DNA from members of the genusSaccharum and related species. DNA yields and purities were similar for allSaccharum species sampled. The method described here resulted in high quality total DNA suitable for polymerase chain reaction (PCR)-based techniques as well as restriction endonuclease digestion, Southern hybridization, and DNA cycle-sequencing. |
| Keywords: | DNA extraction Saccharum complex grasses sugarcane Andropogoneae |
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