HMGA1 is a new target of miR-195 involving isoprenaline-induced cardiomyocyte hypertrophy |
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Authors: | Xiang-Yu You Jiong-Hua Huang Bin Liu Shao-Jun Liu Yun Zhong Shi-Ming Liu |
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Institution: | 1. Cardiovascular Department, Second Hospital Affiliated to Guangzhou Medical University, Guangzhou Institute of Cardiovascular Disease, 250 Chang-gang East Road, Guangzhou, 510260, PR China
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Abstract: | Emerging data have shown that microRNAs (miRNAs) have important functions in the processes of cardiac hypertrophy and heart failure that occur during the postnatal period. Cardiac overexpression of miR-195 results in pathological cardiac growth and heart failure in transgenic mice. In the present study, we analyzed the roles of miR-195 in cardiomyocyte hypertrophy and found that miR-195 was greatly upregulated during isoprenaline-induced cardiomyocyte hypertrophy. By using mRNA microarray and molecular approach, we identified a novel putative target of miR-195 called high-mobility group A1 (HMGA1). Total mRNA microarray showed that HMGA1 was downregulated in primary cardiomyocytes that overexpressed miR-195. Using luciferase activity assay, we demonstrated that miR-195 interacts with the 3′-untranslated region of HMGA1 mRNA. Moreover, we showed that miR-195 in primary cardiomyocytes downregulates the expression of HMGA1 at the protein level. Taken together, our data demonstrated that miR-195 can negatively regulate a new target, HMGA1, which is involved in cardiomyocyte hypertrophy. |
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