| 响应面优化纤维素酶辅助提取木豆叶总黄酮工艺 |
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| 引用本文： | 王秋雪；顾成波；付丽楠；李旺；袁肖寒；祖元刚；付玉杰.响应面优化纤维素酶辅助提取木豆叶总黄酮工艺[J].植物研究,2012,32(6):765-769. |
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| 作者姓名： | 王秋雪；顾成波；付丽楠；李旺；袁肖寒；祖元刚；付玉杰 |
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| 作者单位： | 1.东北林业大学森林植物生态学教育部重点实验室，哈尔滨 150040；;2.东北林业大学林业生物制剂教育部工程研究中心， 哈尔滨 150040；;3.东北农业大学生命科学与生物技术研究中心，哈尔滨 150030 |
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| 基金项目： | 哈尔滨市科技创新人才专项资金(2011RFQXS074);中央高校基本科研业务费专项资金资助(DL09BA20) |
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| 摘 要： | 为探讨纤维素酶辅助提取木豆叶总黄酮最佳工艺条件，在单因素试验基础上，以酶用量、酶解温度、酶解时间和酶解pH为影响因子，总黄酮得率为响应值，采用Box-behnken中心组合设计建立4个影响因子与总黄酮得率关系的数学模型，进行响应面法分析。结果表明，纤维素酶辅助提取木豆叶总黄酮的最佳工艺条件为：酶用量为8.65 mg，酶解温度为33.88℃，酶解时间为2.02 h，酶解pH为5.02。在最优条件下总黄酮理论得率为4.86%，实测值为4.88%，拟合得到的模型与实际吻合良好。本研究建立的提取工艺条件稳定可靠，为以后木豆叶总黄酮的应用开发提供实验依据。
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| 关 键 词： | 木豆 总黄酮 纤维素酶 响应面 |
Optimization of Cellulase-assisted Extraction Process of Total Flavonoids from Pigeon Pea Leaves Using Response Surface Methodology |
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| WANG Qiu-Xue,GU Cheng-Bo,FU Li-Nan,LI Wang,YUAN Xiao-Han,ZU Yuan-Gang,FU Yu-Jie.Optimization of Cellulase-assisted Extraction Process of Total Flavonoids from Pigeon Pea Leaves Using Response Surface Methodology[J].Bulletin of Botanical Research,2012,32(6):765-769. |
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| Authors: | WANG Qiu-Xue GU Cheng-Bo FU Li-Nan LI Wang YUAN Xiao-Han ZU Yuan-Gang FU Yu-Jie |
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| Institution: | 1,2(1.Key Laboratory of Forest Plant Ecology of Northeast Forestry University,Ministry of Education,Harbin 150040;2.Engineering Research Center of Forest Bio-preparation,Ministry of Education,Northeast Forestry University,Harbin 150040;3.Life Science and Biotechnique Research Center,Northeast Agricultural University,Harbin 150030) |
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| Abstract: | Response surface methodology (RSM) was used to optimize the cellulase-assisted extraction process of total flavonoids (TF) from pigeon pea leaves. The amount of enzyme, enzymatic hydrolysis temperature, time and pH were selected as influencing factors and TF yield as the response value based on single factor trials. The experiment mathematical model was established according to Box-Behnken central composite experiment design and was analyzed by RSM. The results showed the optimal extraction conditions were as follows：enzyme amount 8.65 mg, extraction temperature 33.88℃, extraction time 2.02 h and extraction pH 5.02. The predicted value of TF yield was 4.86% under the optimum conditions, and the experimental value was 4.88%. The optimal extraction conditions were stable and reliable, and would provide experimental basis for the future development of TF in pigeon pea leaves. |
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| Keywords: | pigeon pea total flavonoids cellulase response surface methodology |
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