蝴蝶兰离体培养及其相关生物技术研究进展

对蝴蝶兰(Phalaenopsis)的离体培养、褐化及控制、物理化学诱变、转基因研究进展进行了综述,包括蝴蝶兰离体培养中的外植体选择、体细胞胚胎及类原球茎诱导、培养基及培养条件、培养方式、褐化控制等关键因素,并概括了生物反应器、转基因、诱变育种等相关生物技术研究进展。成体植株的花梗、茎尖和种子以及试管苗的叶片、茎尖、根尖等均可作为外植体;影响外植体褐化的主要因素有培养基的种类、pH值、温度、外植体的生理状况等;蝴蝶兰诱变育种及转基因研究尚处于初步阶段。

Research Progress on Phalaenopsis in vitro Culture and Related Biotechnology

The main research progresses on Phalaenopsis in vitro culture were reviewed, including explants selection, embryogenesis and protocorm-like body induction, medium, culture condition and method, and browning control, eg. Meanwhile, the bioreactor, genetic transformation, mutation breeding and other biotechnology were summarized. The flower stalk, tip of shoots and seeds from adult plants were common used as explants, and do the leaf, tip of shoots, root tips from tube plantlets. The main factors of explants browning were type of medium, pH, temperature and the physiological state. The researches about mutational breeding and genetic transformation in Phalaenopsis were still at preliminary stage.