胶体金法与RT-PCR法测定A群轮状病毒及G血清型分型

目的了解婴幼儿A群轮状病毒腹泻的流行病学特征。方法收集浙江省苍南县人民医院2009年1-12月份的婴幼儿腹泻粪便标本,采用胶体金免疫层析法和逆转录-聚合酶链反应法进行轮状病毒抗原检测和血清型分型,分析G血清型分型。结果 828份婴幼儿腹泻粪便轮状病毒阳性率为33.45%。在轮状病毒腹泻患儿中,1岁以内占49.82%,2岁以内占89.89%。本地区轮状病毒腹泻呈现季节高峰,11月至次年1月为轮状病毒腹泻流行期。毒株分型显示G3型为流行优势株（51.6%）,其次是G1型（26.6%）;另有13.7%为混合感染,包括G1＋G3型（10.5%）、G2＋G3型（2.4%）和G1＋G9型（0.8%）。结论 2009年浙江省苍南县婴幼儿轮状病毒腹泻的主要血清型是G3和G1,G3为优势毒株。 更多还原

A study of Rotavirus A by DIGFA,RT-PCR and G-serotyping

Objective To study the prevalent status of infantile rotavirus A diarrhoea.Method Stool samples of infantile diarrhoea from the People′s Hospital of Cangnan County,Zhejiang in 2009 were collected and detected with the method of DIGFA,and the G serotypes were analyzed by the method of RT-PCR.Result Of the 828 samples detected,the positive rate was 33.45%.Among all the RV diarrhoea,49.82% of them were in children less than 1 year old and 89.89% of them in children less than 2 years old.Rotavirus presented a marked seasonal peak form November to January.Rotavirus G-serotyping results showed that G3（51.6%） was the most prevalent serotype followed by G1（26.6%）;Mixed Infection（13.7%） was aslo identified,inciluding G1＋G3（10.5%）,G2＋G3（2.4%） and G1＋G9（0.8%）.Conclusion Rotavirus A was the most important virus strain of infantile diarrhoea in 2009 in Cangnan County,Zhejiang,and the major serotypes were G3 and G1,with G3 being the predominant virulent strain.