Abstract: | General chemical and immunochemical characterization of human embryonic prealbumin-1 (EPA-1) isolated from abortive blood is presented. EPA-1 was found to exist as glycosylated and non-glycosylated forms, which are immunochemically identical. Sugar moiety of the glycosylated form contains residues of fucose (3.0%), mannose (3.2%), galactose (7.8%), N-acetylglucosamine (5.4%), N-acetylgalactosamine (1.2%) and N-acetylneuraminic acid. Using methylation studies, types of bonds between the sugar residues were elucidated. |