Modified method of mammalian cell synchronization improves yield and degree of synchronization |
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Authors: | Guanghui Cao Li-Ming Liu Stephen F. Cleary |
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Affiliation: | Physiology Department, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298. |
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Abstract: | A modified method to synchronize CHO and HeLa cells is developed based upon a combined shaking-off and chemical blockage. This method has effectively blocked quiescent cells, which is the main obstacle of high degree synchronization. Flow-cytometry data show the improvement on the degree of synchronization and yield compared to two previously used methods. |
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