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Vanadium-containing tunicate blood cells are not highly acidic
Authors:Amy L. Dingley   Kenneth Kustin   Ian G. Macara   Guy C. Mcleod  Mary F. Roberts
Affiliation:1. Department of Chemistry, Brandeis University, Waltham, MA 02254 U.S.A.;2. Department of Biochemistry and Molecular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138 U.S.A.;3. New England Aquarium, Central Wharf, Boston, MA 02110 U.S.A.;4. Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139 U.S.A.
Abstract:The intracellular pH of intact blood cells of the tunicate Ascidia nigra was measured by transmembrane equilibration of [14C]methylamine. The pH of unfractionated blood cells is 7.39±0.10. The pH of vanadocytes, determined in a fractionation study, is 7.2. Previously used methods, in which pH values less than 3.0 are inferred from cell lysis or vital staining experiments, are shown to be unsuitable for intracellular pH determination due to the chemical composition of these vanadium-containing cells.
Keywords:Vanadium   Methylamine   Transmembrane equilibration   Intracellular pH   (Tunicate blood cell)
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