Vanadium-containing tunicate blood cells are not highly acidic |
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| Authors: | Amy L Dingley Kenneth Kustin Ian G Macara Guy C Mcleod Mary F Roberts |
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| Institution: | 1. Department of Chemistry, Brandeis University, Waltham, MA 02254 U.S.A.;2. Department of Biochemistry and Molecular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138 U.S.A.;3. New England Aquarium, Central Wharf, Boston, MA 02110 U.S.A.;4. Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139 U.S.A. |
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| Abstract: | The intracellular pH of intact blood cells of the tunicate Ascidia nigra was measured by transmembrane equilibration of 14C]methylamine. The pH of unfractionated blood cells is 7.39±0.10. The pH of vanadocytes, determined in a fractionation study, is 7.2. Previously used methods, in which pH values less than 3.0 are inferred from cell lysis or vital staining experiments, are shown to be unsuitable for intracellular pH determination due to the chemical composition of these vanadium-containing cells. |
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| Keywords: | Vanadium Methylamine Transmembrane equilibration Intracellular pH (Tunicate blood cell) |
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